作者：杨小红， 袁江，周远明， 张瑜， 曾亚翎， 刘谋治， 赵冰清
【摘要】 目的探讨白首乌（耳叶牛皮消Cynanchum auriculatum Royle ex Wight）多糖对小鼠肝损伤的保护作用。方法从白首乌（耳叶牛皮消）中提取粗多糖，将84只健康小鼠动物随机分组，随机分为模型组、生理盐水对照组、阳性对照组和3个实验对照组。实验组每组14只按低剂量组（100 mg·kg-1·d-1）、中剂量组（250 mg·kg-1·d-1）、高剂量组（500 mg·kg-1·d-1）连续灌胃多糖8 d，对照组按等量生理盐水灌胃。通过摘眼球取血测量谷丙转氨酶（AST）、谷草转氨酶（ALT）含量。结果白首乌多糖肝损伤防治组小鼠血清ALT及AST活性比模型组显著降低，两组比较，差异有统计学意义。结论 白首乌多糖对酒精所致小鼠急性肝损伤模型具有显著的保护作用。
【关键词】 耳叶牛皮消； 粗多糖； 酒精； 肝损伤
Abstract：ObjectiveTo investigate the protective effect of polysaccharide from Bai-shou-wu (Cynanchum auriculatum Royle ex Wight) on alcohol-induced liver injury.MethodsIn this experiment,rough polysaccharide from Bai Shou Wu was extracted and 84 mice were randomly divided into normal control groups,model control group, positive control group and three experiment groups.Each experiment group had 14 mice and were fed polysaccharide for 8 days by gavage with respectively high-dosage of 100 mg/(kg·d),middle-dosage of 250 mg/(kg·d) and low-dosage of 500 mg/(kg·d),while the control groups were given the same volume of physiological saline.The concentration of the serum GPT/ALT and GOT/AST were measured by extracting the eyeball blood.ResultsThe activity of the serum GPT/ALT and GOT/AST in the experiment group decreased more significantly than that in model control group,and the differences were statically significant.ConclusionPolysaccharide from Cynanchum auriculatum Royle ex Wight has significant protective effect on ethanol-induced liver injury in mice.
Key words：Cynanchum auriculatum Royle ex Wight; Polysaccharide; Alcohol; Liver injury
白首乌为祖国传统中药，其主要来源为萝藦科植物耳叶牛皮消Cynanchum auriculatum Royle ex Wight，隔山消Cynanchum wilfordii (Maxim.)Hemsl和戟叶牛皮消Cynanchum bungei Decne.的块根，具有养血益肝、固肾益精、乌须黑发和延年益寿的功效［1，2］。现代研究发现白首乌中含有多种有效成分，具有抗肿瘤、抗衰老和保肝等多方面的药理活性［1，3］。国外的研究也证实了这些药理活性［4～6］。
1 器材
1.1 药品与试剂
白首乌购自江苏滨海，经湖南省药检所方石林教授鉴定为萝摩科植物耳叶牛皮消Cynanchum auriculatum Royle ex Wight，实验用酒精（AR），谷草转氨酶(ALT)试剂盒(南京建成生物工程研究所)。谷丙转氨酶酶(AST)试剂盒(南京建成生物工程研究所)。氯仿、丙酮、乙醚、正丁醇均为分析纯。护肝片为北京亚东生物制药有限公司生产。
1.2 仪器与设备
电热恒温干燥箱（天津市泰斯特仪器有限公司）202－3AB型；电热恒温水浴锅（天津市泰斯特仪器有限公司）DK－98-1A；真空旋转蒸发器（上海亚荣生化仪器厂）RE 52－99；抽滤机（郑州长城科工贸有限公司）SHB－B95型；离心沉淀机（上海医用分析仪器厂）LXT－II；分析天平（湘仪天平仪器厂）TG328B。
